In silico Epitope Prediction and Immunogenic Analysis for Penton base Epitope-focused Vaccine against Hydropericardium syndrome in chicken

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In silico Epitope Prediction and Immunogenic Analysis for Penton base Epitope-focused Vaccine against Hydropericardium syndrome in chicken. / Aziz, Faiza; Tufail, Soban; Shah, Majid Ali; Salahuddin Shah, Muhammad; Habib, Mudasser; Mirza, Osman; Iqbal, Mazhar; Rahman, Moazur.

I: Virus Research, 08.09.2019, s. 197750.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Aziz, F, Tufail, S, Shah, MA, Salahuddin Shah, M, Habib, M, Mirza, O, Iqbal, M & Rahman, M 2019, 'In silico Epitope Prediction and Immunogenic Analysis for Penton base Epitope-focused Vaccine against Hydropericardium syndrome in chicken', Virus Research, s. 197750. https://doi.org/10.1016/j.virusres.2019.197750

APA

Aziz, F., Tufail, S., Shah, M. A., Salahuddin Shah, M., Habib, M., Mirza, O., ... Rahman, M. (2019). In silico Epitope Prediction and Immunogenic Analysis for Penton base Epitope-focused Vaccine against Hydropericardium syndrome in chicken. Virus Research, 197750. https://doi.org/10.1016/j.virusres.2019.197750

Vancouver

Aziz F, Tufail S, Shah MA, Salahuddin Shah M, Habib M, Mirza O o.a. In silico Epitope Prediction and Immunogenic Analysis for Penton base Epitope-focused Vaccine against Hydropericardium syndrome in chicken. Virus Research. 2019 sep 8;197750. https://doi.org/10.1016/j.virusres.2019.197750

Author

Aziz, Faiza ; Tufail, Soban ; Shah, Majid Ali ; Salahuddin Shah, Muhammad ; Habib, Mudasser ; Mirza, Osman ; Iqbal, Mazhar ; Rahman, Moazur. / In silico Epitope Prediction and Immunogenic Analysis for Penton base Epitope-focused Vaccine against Hydropericardium syndrome in chicken. I: Virus Research. 2019 ; s. 197750.

Bibtex

@article{ec1ce766ca0b4702b89170c762a03b8f,
title = "In silico Epitope Prediction and Immunogenic Analysis for Penton base Epitope-focused Vaccine against Hydropericardium syndrome in chicken",
abstract = "Certain strains of fowl adenovirus serotype 4 (FAdV-4) of the family Adenoviridae are recognized to be the causative agents of Hydropericardium Syndrome (HPS) in broiler chicken. Despite the significantly spiking mortality in broilers due to HPS, not much effort has been made to design an effective vaccine against FAdV-4. The combination of immuno- and bio-informatics tools for immunogenic epitope prediction is the most recent concept of vaccine design. It reduces the time and effort required for hunting a potent vaccine candidate and is economical. Previously, we have reported the penton base protein of FAdV-4 to be a candidate for subunit vaccine against HPS. And in the present study, we have computationally pre-screened promising B- and T-cell epitopes of the penton base. Multiple methods were employed for linear B-cell epitope identification; BepiPred and five other methods based on physicochemical properties of the amino acids. The penton base was homology modeled by means of Modeller 9.17 and after refinement of the model (by GalaxyRefine web server) ElliPro web tool was used to predict the discontinuous epitopes. NetMHCcons 1.1 and NetMHCIIpan 3.1 servers were used for the likelihood of peptide binding to Major Histocompatibility Complex (MHC) class I & II molecules respectively for T-cell epitope forecast. As a result, we identified the peptide stretch of 1-225 as the most promiscuous B- and T-cell epitope region in penton base Full Length (FL) protein sequence. Escherichia coli based expression vectors were generated containing cloned peptide stretch 1-225 (penton base1-225) and penton base FL gene sequence. The recombinant penton base1-225 and penton base FL proteins were expressed and purified using Escherichia coli-based expression system. Purification yield of penton base1-225 was 3-fold higher compared to penton base FL. These proteins were injected in chickens to determine their competence in protection against HPS. The results showed equal protection level of the two proteins and commercial inactivated vaccine against FAdV-4 infection. The results suggest the peptide stretch 1-225 of penton base as a valuable candidate for developing an epitope-driven vaccine to combat HPS.",
author = "Faiza Aziz and Soban Tufail and Shah, {Majid Ali} and {Salahuddin Shah}, Muhammad and Mudasser Habib and Osman Mirza and Mazhar Iqbal and Moazur Rahman",
note = "Copyright {\circledC} 2019. Published by Elsevier B.V.",
year = "2019",
month = "9",
day = "8",
doi = "10.1016/j.virusres.2019.197750",
language = "English",
pages = "197750",
journal = "Virus Research",
issn = "0168-1702",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - In silico Epitope Prediction and Immunogenic Analysis for Penton base Epitope-focused Vaccine against Hydropericardium syndrome in chicken

AU - Aziz, Faiza

AU - Tufail, Soban

AU - Shah, Majid Ali

AU - Salahuddin Shah, Muhammad

AU - Habib, Mudasser

AU - Mirza, Osman

AU - Iqbal, Mazhar

AU - Rahman, Moazur

N1 - Copyright © 2019. Published by Elsevier B.V.

PY - 2019/9/8

Y1 - 2019/9/8

N2 - Certain strains of fowl adenovirus serotype 4 (FAdV-4) of the family Adenoviridae are recognized to be the causative agents of Hydropericardium Syndrome (HPS) in broiler chicken. Despite the significantly spiking mortality in broilers due to HPS, not much effort has been made to design an effective vaccine against FAdV-4. The combination of immuno- and bio-informatics tools for immunogenic epitope prediction is the most recent concept of vaccine design. It reduces the time and effort required for hunting a potent vaccine candidate and is economical. Previously, we have reported the penton base protein of FAdV-4 to be a candidate for subunit vaccine against HPS. And in the present study, we have computationally pre-screened promising B- and T-cell epitopes of the penton base. Multiple methods were employed for linear B-cell epitope identification; BepiPred and five other methods based on physicochemical properties of the amino acids. The penton base was homology modeled by means of Modeller 9.17 and after refinement of the model (by GalaxyRefine web server) ElliPro web tool was used to predict the discontinuous epitopes. NetMHCcons 1.1 and NetMHCIIpan 3.1 servers were used for the likelihood of peptide binding to Major Histocompatibility Complex (MHC) class I & II molecules respectively for T-cell epitope forecast. As a result, we identified the peptide stretch of 1-225 as the most promiscuous B- and T-cell epitope region in penton base Full Length (FL) protein sequence. Escherichia coli based expression vectors were generated containing cloned peptide stretch 1-225 (penton base1-225) and penton base FL gene sequence. The recombinant penton base1-225 and penton base FL proteins were expressed and purified using Escherichia coli-based expression system. Purification yield of penton base1-225 was 3-fold higher compared to penton base FL. These proteins were injected in chickens to determine their competence in protection against HPS. The results showed equal protection level of the two proteins and commercial inactivated vaccine against FAdV-4 infection. The results suggest the peptide stretch 1-225 of penton base as a valuable candidate for developing an epitope-driven vaccine to combat HPS.

AB - Certain strains of fowl adenovirus serotype 4 (FAdV-4) of the family Adenoviridae are recognized to be the causative agents of Hydropericardium Syndrome (HPS) in broiler chicken. Despite the significantly spiking mortality in broilers due to HPS, not much effort has been made to design an effective vaccine against FAdV-4. The combination of immuno- and bio-informatics tools for immunogenic epitope prediction is the most recent concept of vaccine design. It reduces the time and effort required for hunting a potent vaccine candidate and is economical. Previously, we have reported the penton base protein of FAdV-4 to be a candidate for subunit vaccine against HPS. And in the present study, we have computationally pre-screened promising B- and T-cell epitopes of the penton base. Multiple methods were employed for linear B-cell epitope identification; BepiPred and five other methods based on physicochemical properties of the amino acids. The penton base was homology modeled by means of Modeller 9.17 and after refinement of the model (by GalaxyRefine web server) ElliPro web tool was used to predict the discontinuous epitopes. NetMHCcons 1.1 and NetMHCIIpan 3.1 servers were used for the likelihood of peptide binding to Major Histocompatibility Complex (MHC) class I & II molecules respectively for T-cell epitope forecast. As a result, we identified the peptide stretch of 1-225 as the most promiscuous B- and T-cell epitope region in penton base Full Length (FL) protein sequence. Escherichia coli based expression vectors were generated containing cloned peptide stretch 1-225 (penton base1-225) and penton base FL gene sequence. The recombinant penton base1-225 and penton base FL proteins were expressed and purified using Escherichia coli-based expression system. Purification yield of penton base1-225 was 3-fold higher compared to penton base FL. These proteins were injected in chickens to determine their competence in protection against HPS. The results showed equal protection level of the two proteins and commercial inactivated vaccine against FAdV-4 infection. The results suggest the peptide stretch 1-225 of penton base as a valuable candidate for developing an epitope-driven vaccine to combat HPS.

U2 - 10.1016/j.virusres.2019.197750

DO - 10.1016/j.virusres.2019.197750

M3 - Journal article

SP - 197750

JO - Virus Research

JF - Virus Research

SN - 0168-1702

ER -

ID: 227187607