Purification, crystallization and preliminary X-ray diffraction of the G3BP1 NTF2-like domain
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
The nuclear transport factor 2-like (NTF2-like) domain of human G3BP1 was subcloned, overexpressed in Escherichia coli and purified. Crystals were obtained using the hanging-drop vapour-diffusion method. Diffraction data were collected to 3.6 Å resolution using synchrotron radiation. The crystals belonged to the hexagonal space group P6(3)22, with unit-cell parameters a=b=89.84, c=70.02 Å. The crystals contained one molecule per asymmetric unit, with an estimated solvent content of 56%. Initial phases were obtained by molecular replacement.
Originalsprog | Engelsk |
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Tidsskrift | Acta Crystallographica. Section F: Structural Biology and Crystallization Communications Online |
Vol/bind | 67 |
Udgave nummer | Pt 1 |
Sider (fra-til) | 48-50 |
ISSN | 1744-3091 |
DOI | |
Status | Udgivet - 1 jan. 2011 |
- Det tidligere Farmaceutiske Fakultet
Forskningsområder
ID: 33812118