DNA of Dientamoeba fragilis detected within surface-sterilized eggs of Enterobius vermicularis
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DNA of Dientamoeba fragilis detected within surface-sterilized eggs of Enterobius vermicularis. / Röser, Dennis; Nejsum, Peter; Carlsgart, Anne Josefine; Nielsen, Henrik Vedel; Stensvold, Christen Rune.
I: Experimental Parasitology, Bind 133, Nr. 1, 2013, s. 57-61.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - DNA of Dientamoeba fragilis detected within surface-sterilized eggs of Enterobius vermicularis
AU - Röser, Dennis
AU - Nejsum, Peter
AU - Carlsgart, Anne Josefine
AU - Nielsen, Henrik Vedel
AU - Stensvold, Christen Rune
N1 - Copyright © 2012. Published by Elsevier Inc.
PY - 2013
Y1 - 2013
N2 - With no evidence of a cyst stage, the mode of transmission of Dientamoeba fragilis, an intestinal protozoon of common occurrence and suggested pathogenicity, is incompletely known. Numerous studies have suggested that eggs of intestinal nematodes, primarily Enterobius vermicularis (pinworm), can serve as vectors for D. fragilis, although attempts to culture D. fragilis from pinworm eggs have been unsuccessful and data from epidemiological studies on D. fragilis/pinworm co-infection have been conflicting. The aim of this study was to investigate whether we could detect D. fragilis DNA from pinworm eggs collected from routine diagnostic samples (cellophane tape) and surface-sterilised by hypochlorite. DNA was extracted from individual eggs and tested by PCR using D. fragilis- and E. vermicularis-specific primers; amplicons were sequenced for confirmation. In cellophane tape samples from 64 patients with unknown D. fragilis status we detected D. fragilis DNA in 12/238 (5%) eggs, and in a patient known to harbour D. fragilis we detected D. fragilis DNA in 39/99 (39%) eggs. The finding of D. fragilis DNA within eggs of E. vermicularis strongly supports the hypothesis of D. fragilis-transmission by pinworm and has implications for antimicrobial intervention as well as control and public health measures.
AB - With no evidence of a cyst stage, the mode of transmission of Dientamoeba fragilis, an intestinal protozoon of common occurrence and suggested pathogenicity, is incompletely known. Numerous studies have suggested that eggs of intestinal nematodes, primarily Enterobius vermicularis (pinworm), can serve as vectors for D. fragilis, although attempts to culture D. fragilis from pinworm eggs have been unsuccessful and data from epidemiological studies on D. fragilis/pinworm co-infection have been conflicting. The aim of this study was to investigate whether we could detect D. fragilis DNA from pinworm eggs collected from routine diagnostic samples (cellophane tape) and surface-sterilised by hypochlorite. DNA was extracted from individual eggs and tested by PCR using D. fragilis- and E. vermicularis-specific primers; amplicons were sequenced for confirmation. In cellophane tape samples from 64 patients with unknown D. fragilis status we detected D. fragilis DNA in 12/238 (5%) eggs, and in a patient known to harbour D. fragilis we detected D. fragilis DNA in 39/99 (39%) eggs. The finding of D. fragilis DNA within eggs of E. vermicularis strongly supports the hypothesis of D. fragilis-transmission by pinworm and has implications for antimicrobial intervention as well as control and public health measures.
KW - Former LIFE faculty
KW - Vector
KW - Protozoon
KW - Nematode
KW - Parasite
KW - PCR
KW - Transmission
U2 - 10.1016/j.exppara.2012.10.009
DO - 10.1016/j.exppara.2012.10.009
M3 - Journal article
C2 - 23116599
VL - 133
SP - 57
EP - 61
JO - Experimental Parasitology
JF - Experimental Parasitology
SN - 0014-4894
IS - 1
ER -
ID: 41933555