Next-generation sequencing technology for genetics and genomics of sorghum
Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
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Next-generation sequencing technology for genetics and genomics of sorghum. / Luo, Hong; Mocoeur, Anne Raymonde Joelle; Jing, Hai-Chun.
Genetics, genomics and breeding of sorghum. red. / Yi-Hong Wang; Hari D. Upadhyaya; Chittaranjan Kole. CRC Press, 2014. s. 226-250.Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
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TY - CHAP
T1 - Next-generation sequencing technology for genetics and genomics of sorghum
AU - Luo, Hong
AU - Mocoeur, Anne Raymonde Joelle
AU - Jing, Hai-Chun
PY - 2014
Y1 - 2014
N2 - The invention and application of Next-Generation Sequencing (NGS) technologies have revolutionized the study of genetics and genomics. Much research which would not even be considered are nowdays being excuted in many laboratories as routine. In this chapter, we introduce the currently available NGS platforms, comparing their working theories and reveiwing their advantages and disavantages. We also discuss the future of NGS development and point out that single molecular sequencing would push the technology to the next level for biological sciences. Much of the chapter focuses on the use of NGS technologies in sorghum. Although the acquisition of the first whole-genome sequence in sorghum was carried out primarily using Sanger sequencing, the use of NGS for examining the genome-wide variation was almost synchronized with other work. Interesting genomic variation was found between sweet and grain sorghum. NGS has also been used to examine the transcriptomes of sorghum under various stress conditions. Besides identifying interesting transcriptonal adpatation to stress conditions, these study show that sugar could potentially act as an osmitic adjusting factor via transcriptional regulation. Furthermore, miRNAs are found to be important adaptation to both biotic and abiotic stresses in sorghum. We discuss the use of NGS for further genetic improvement and breeding in sorghum.
AB - The invention and application of Next-Generation Sequencing (NGS) technologies have revolutionized the study of genetics and genomics. Much research which would not even be considered are nowdays being excuted in many laboratories as routine. In this chapter, we introduce the currently available NGS platforms, comparing their working theories and reveiwing their advantages and disavantages. We also discuss the future of NGS development and point out that single molecular sequencing would push the technology to the next level for biological sciences. Much of the chapter focuses on the use of NGS technologies in sorghum. Although the acquisition of the first whole-genome sequence in sorghum was carried out primarily using Sanger sequencing, the use of NGS for examining the genome-wide variation was almost synchronized with other work. Interesting genomic variation was found between sweet and grain sorghum. NGS has also been used to examine the transcriptomes of sorghum under various stress conditions. Besides identifying interesting transcriptonal adpatation to stress conditions, these study show that sugar could potentially act as an osmitic adjusting factor via transcriptional regulation. Furthermore, miRNAs are found to be important adaptation to both biotic and abiotic stresses in sorghum. We discuss the use of NGS for further genetic improvement and breeding in sorghum.
KW - Former LIFE faculty
KW - Sorghum bicolour L.; Quality Declared Seed System
KW - Next generation sequencing
KW - Genetic
KW - genomic variation
KW - molecular breeding
KW - biofuel-associated traits
KW - Association analysis
M3 - Book chapter
SN - 978-1-4822-1008-8
SP - 226
EP - 250
BT - Genetics, genomics and breeding of sorghum
A2 - Wang, Yi-Hong
A2 - Upadhyaya, Hari D.
A2 - Kole, Chittaranjan
PB - CRC Press
ER -
ID: 118579214